Evaluated Characteritics of Chicken Bone Marrow-derived Dendritic Cells Following LPS Induced Matura

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Dendritic cells (DCs) are bone marrow-derived professional antigen presenting cells (APCs),they are crucial for initiation of both innate and adaptive immune responses.In this study,chicken bone marrow (chBM) cells were cultured in medium with recombinant chicken granulocyte-macrophage colony stimulating factor (rGM-CSF) and recombinant chicken interleukin-4 (rIL-4)for 7 days,displayed the typical morphology of DCs.These immature chicken bone marrow-derived DCs (chBM-DCs) showed significant up-regulation of the putative CD1 1c and of major histocompatibility complex class II (MHC Ⅱ),but CD40 and CD86 co-stimulatory molecules were almost no up-regulated.However,maturation with lipopolysaccharide (LPS),surface expression of CD40,CD86 was greatly increased.The phagocytosis of chBM-DCs was assessed by neutral red,and the phagocytosis decreased after stimulation.In mixed lymphocyte responses (MLR),stimulated chBM-DCs were more effective to T-cell stimulators than nonstimulated chBM-DCs.In addition,mRNA expression levels of IL-lβ,IL-4,IL-6,IL-10,IL-12,IFN-γ,TNF-α,CXCLil and CXCLi2 were assessed by real-time qPCR (qRT-PCR),and the results showed cultured chBM-DCs could be matured to a T helper cell type 1 (Th 1)-promoting phenotype by LPS stimulation.
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