本文用健康人血对 CB 微核法进行了探讨,发现用全血、1ml 培养体系、准确滴加 Cyt-B 的方法,比由全血分离淋巴细胞的方法省时、省血,且细胞不易成堆,破坏小,不需二氧化碳孵箱设备。诱发大量双核 CB 细胞的最适浓度为6μg/ml,虽 Cyt-B 用量比文献报道的稍大,但节省了其它试剂,仍值得选择使用。 In this paper, healthy human blood CB micronucleus was explored and found that the whole blood, 1ml culture system, accurate drop of Cyt-B method than the whole blood separation of lymphocytes save time, save blood, and the cell is not easy Piles, destruction of small, without carbon dioxide incubator equipment. The optimal concentration for inducing a large amount of binuclear CB cells was 6μg / ml. Although the dosage of Cyt-B was slightly larger than that reported in the literature, other reagents were saved and it is still worth to choose to use.