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目的:优选匙羹藤中牛弥菜醇A的提取工艺条件。方法:以牛弥菜醇A的含量为指标,通过不同提取方法的比较选择合适的提取方法;采用L9(34)正交试验,考察乙醇浓度、乙醇用量和浸泡时间对提取效果的影响。结果:最佳提取工艺为8倍量75%乙醇渗漉提取,浸泡24h。结论:该工艺合理可行,稳定可靠,可为牛弥菜醇A的工业化提取提供实验依据。
OBJECTIVE: To optimize the extraction conditions of cattleidin A in Gymnema sylvestre. Methods: With the content of bovine serum albumin A as the index, the suitable extraction method was selected by comparison of different extraction methods. The effects of ethanol concentration, ethanol dosage and soaking time on extraction were investigated by L9 (34) orthogonal test. Results: The best extraction process for the 8 times the amount of 75% ethanol percolation extract, soak 24h. Conclusion: The process is reasonable and feasible, stable and reliable, which can provide experimental evidence for the industrial extraction of bovine tropine A.