目的对沉香叶的急性经口毒性、遗传毒性进行研究,为沉香叶的开发和应用提供毒理学安全性实验依据。方法按照GB 15193—2003对沉香叶进行小鼠经口急性毒性试验(Horn’s法,设21.5 g/kg体重、10.0 g/kg体重、4.64 g/kg体重、2.15 g/kg体重4个剂量组)、小鼠精子畸形试验(设10.0 g/kg体重、5.00 g/kg体重、2.50 g/kg体重3个剂量组及阴性、阳性对照组)、小鼠骨髓细胞微核试验(设10.0 g/kg体重、5.00 g/kg体重、2.50 g/kg体重3个剂量组及阴性、阳性对照组)及鼠伤寒沙门菌回复突变试验(设5 000μg/皿、1 000μg/皿、200μg/皿、40μg/皿、8μg/皿5个剂量组,同时设自发回变、溶剂对照和阳性对照组)。结果沉香叶对小鼠的急性经口LD50>21.5 g/kg体重;各剂量组微核率、精子畸形率、回复突变数和阴性对照组比较均无显著性增加;3项毒理学遗传试验结果均为阴性。结论在本实验条件下,沉香叶属于无毒级物质,未见有遗传毒性作用。 OBJECTIVE To study the acute oral toxicity and genotoxicity of Anemarrhenae asphodeloides leaves and to provide evidence for toxicology and safety for the development and application of Anemones. Methods According to GB 15193-2003, the mice were subjected to oral acute toxicity test (Horn’s method, with 21.5 g / kg body weight, 10.0 g / kg body weight, 4.64 g / kg body weight and 2.15 g / kg body weight 4 doses) , Mice sperm deformity test (10.0 g / kg body weight, 5.00 g / kg body weight, 2.50 g / kg body weight three dose groups and negative, positive control group), mouse bone marrow cell micronucleus test (5 000 μg / dish, 1 000 μg / dish, 200 μg / dish, 40 μg / dish), and the control group Dish, 8μg / dish five dose group, set spontaneous regression, solvent control and positive control group). Results The acute oral LD50> 21.5 g / kg body weight of mice was not significantly increased with the frequency of micronucleus, the rate of sperm deformity, the number of back mutations and the negative control group. The results of three toxicological genetic tests All negative. Conclusion Under the experimental conditions, lancea leaves are non-toxic substances, no genotoxic effects were observed.