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目的 观察血管内皮生长因子(VEGF)-C在胰腺癌组织内的表达情况,分析VEGF-C的表达与胰腺癌淋巴结转移和预后之间的关系。方法 取胰腺癌病例52例,其中,伴淋巴结转移组36例,无淋巴结转移组16例。应用免疫组化法和Western blot技术观察VEGF-C在胰腺癌组织内的表达。以D2-40作为淋巴管内皮特异性标记物,观察胰腺癌组织内淋巴管生成的情况。采用Kaplan-Meier法绘制生存曲线判断VEGF-C的表达对胰腺癌预后的影响。结果 Western blot和免疫组化法检测结果表明,VEGF-C主要表达于胰腺癌细胞浆内,淋巴结转移组阳性表达量明显高于无淋巴结转移组(p<0.05)。D2-40表达于胰腺癌组织内淋巴管内皮细胞,VEGF-C阳性组淋巴管数密度明显高于VEGF-C阴性组(p<0.05),表明VEGF-C的表达与胰腺癌淋巴管生成密切相关。Kaplan-Meier生存分析表明VEGF-C表达阴性患者的生存率均高于VEGF-C表达阳性患者,VEGF-C的表达影响患者的预后。结论 VEGF-C在胰腺癌的淋巴管生成和淋巴结转移过程中发挥重要作用,VEGF-C的表达是影响胰腺癌患者预后的主要因素之一。
Objective To observe the expression of vascular endothelial growth factor (VEGF) -C in pancreatic cancer tissues and to analyze the relationship between the expression of VEGF-C and lymph node metastasis and prognosis of pancreatic cancer. Methods Pancreatic cancer cases were taken in 52 cases, including 36 cases with lymph node metastasis and 16 cases without lymph node metastasis. The expression of VEGF-C in pancreatic cancer tissues was observed by immunohistochemistry and Western blot. Using D2-40 as a specific marker for lymphatic endothelial cells, we observed lymphangiogenesis in pancreatic cancer tissues. Kaplan-Meier survival curve drawn to determine the expression of VEGF-C on the prognosis of pancreatic cancer. Results The results of Western blot and immunohistochemistry showed that VEGF-C was mainly expressed in the cytoplasm of pancreatic cancer cells. The positive expression of VEGF-C in lymph node metastasis group was significantly higher than that in non-lymph node metastasis group (p <0.05). D2-40 expression in lymphatic endothelial cells of pancreatic cancer, VEGF-C positive number of lymphatic vessels was significantly higher than VEGF-C negative group (p <0.05), indicating that the expression of VEGF-C and pancreatic cancer lymphangiogenesis close Related. Kaplan-Meier survival analysis showed that the survival rate of patients with negative VEGF-C expression was higher than those with positive VEGF-C expression, and the expression of VEGF-C affected the prognosis of patients. Conclusion VEGF-C plays an important role in lymphangiogenesis and lymph node metastasis of pancreatic cancer. The expression of VEGF-C is one of the main factors affecting the prognosis of pancreatic cancer.