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目的:研究大连紫海胆棘壳色素的体外免疫调节和抗炎活性。方法:以LPS(细菌脂多糖)为炎症诱导物,研究棘壳色素对RAW264.7细胞分泌多种炎症因子的影响。分别采用MTT法及中性红吞噬法研究棘壳色素对脾淋巴细胞增殖及腹腔巨噬细胞吞噬功能的影响。采用补体结合法考察棘壳色素对补体活性的影响。结果:棘壳色素可显著抑制LPS刺激后RAW264.7细胞合成分泌NO(作用质量浓度5~30μg/m L)、IL-6、TNF-α(作用质量浓度60μg/m L)、PGE2及6-KETO-PGF1α(作用质量浓度10~60μg/m L)的水平,但不影响IL-4的分泌水平。20,50μg/m L的棘壳色素均可促进脾淋巴细胞的增殖,30,60μg/m L的棘壳色素可显著增强腹腔巨噬细胞的吞噬活性,1~500μg/m L的棘壳色素还可显著抑制补体活性。结论:海胆棘壳色素可在功能性产品开发方面具有一定应用潜力。
Objective: To study the immunomodulatory and anti-inflammatory activities of Echinocular Pigment in purple sea water of Dalian. Methods: LPS (bacterial lipopolysaccharide) was used as an inducer of inflammation to study the effects of spathema pigment on the secretion of various inflammatory factors in RAW264.7 cells. MTT assay and neutral red-phagocytosis were used to study the effects of spathells on splenic lymphocyte proliferation and peritoneal macrophage phagocytosis. The complement fixation method was used to investigate the effect of the chromogen on the complement activity. Results: Echinocandine pigment could significantly inhibit NO production (5 ~ 30μg / ml), IL-6 and TNF-α (60μg / ml) -KETO-PGF1α (effect concentration of 10 ~ 60μg / m L) levels, but did not affect the level of IL-4 secretion. 20, 50μg / m L of spinous plant pigment can promote the proliferation of splenic lymphocytes, 30,60μg / m L of chromophores can significantly enhance the phagocytic activity of peritoneal macrophages, 1 ~ 500μg / m L of chromophores Can also significantly inhibit complement activity. Conclusion: Sea urchin shell pigment can have certain potential in functional product development.