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目的:建立GC法测定降香药材中反式苦橙油醇及4个氧化苦橙油醇异构体的含量。方法:采用HP-INNAWAX(30 m×0.25 mm×0.25μm)色谱柱,程序升温,进样口温度230℃,流速1.5 m L·min-1;FID检测器,温度250℃;载气为氮气。结果:反式苦橙油醇及氧化苦橙油醇A、B、C、D的线性范围分别为4.57~761.20 ng(r=0.9971)、1.95~146.55 ng(r=0.9988)、4.46~743.80 ng(r=0.9972)、2.17~406.30 ng(r=0.9999)、0.46~190.85 ng(r=0.9971);回收率(n=6)分别为95.4%、98.2%、98.7%、100.6%、100.4%;样品中上述5个成分的含量范围分别为0.90~16.61、0.60~7.61、1.14~8.75、0.24~2.19、0.12~1.34 mg·g-1。结论:降香中5个成分的含量差别较大,且不同产地之间的含量差别也较大,需要有效控制,该方法简便、准确,能为降香质量控制提供依据。
OBJECTIVE: To establish a GC method for the determination of trans-bittergyl alcohol alcohol and 4 oxidized bittern oil isomers of alpinia officinalis. Methods: HP-INNAWAX (30 m × 0.25 mm × 0.25 μm) column was used to program the temperature. The inlet temperature was 230 ℃ and the flow rate was 1.5 m L · min-1. The FID detector was used at 250 ℃. . RESULTS: The linear ranges of A, B, C and D for trans-octreotide and octreotide were 4.57-761.20 ng (r = 0.9971), 1.95-146.55 ng (r = 0.9988) and 4.46-743.80 ng (r = 0.9972), 2.17-406.30 ng (r = 0.9999) and 0.46-190.85 ng (r = 0.9971), respectively. The recoveries were 95.4%, 98.2%, 98.7%, 100.6% and 100.4%, respectively. The content of the above five components in the sample ranged from 0.90 to 16.61, from 0.60 to 7.61, from 1.14 to 8.75, from 0.24 to 2.19 and from 0.12 to 1.34 mg · g -1, respectively. Conclusion: There are great differences in the contents of 5 constituents in Rhizoma et Radix et Rhizoma Rhei, and the differences in contents between different habitats are also large, which need effective control. This method is simple and accurate and can provide the basis for the quality control of Rhizoma.