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目的:阐明幽门螺杆菌(HP)感染伴胃黏膜癌前病变与自噬之间的关系。方法:纳入中山大学附属第五医院消化科胃镜及黏膜下活检确诊为消化性溃疡患者60例,所有患者均采用半定量RT-PCR方法、Western blotting方法检测自噬因子LC3B表达水平,同时行碳14呼气试验进行HP检测,比较HP感染的消化性溃疡伴胃癌前病变与自噬因子LC3B表达水平之间的相关度。结果:LC3B相对表达量与患者的年龄、性别、发病的部位及黏膜损伤(受累范围)的大小差异无统计学意义(P>0.05);而HP感染、胃上皮不典型增生、慢性萎缩性胃炎、肠上皮化生与LC3B的相对表达量具有统计学意义(P<0.05)。结论:自噬因子LC3B mRNA及蛋白高表达与HP感染及消化性溃疡伴胃黏膜不典型增生具有相关性。
Objective: To clarify the relationship between Helicobacter pylori (HP) infection and gastric mucosal precancerous lesions and autophagy. Methods: Sixty patients with peptic ulcer diagnosed by gastroscopy and submucosal biopsy were enrolled in the Department of Gastroenterology, the Fifth Affiliated Hospital of Sun Yat-sen University. All patients were examined by semi-quantitative RT-PCR and Western blotting to detect the expression of autophagy factor LC3B, 14 breath test HP detection, HP infection of peptic ulcer with gastric precancerous lesions and autophagy factor LC3B expression level between the correlation. Results: There was no significant difference in the relative expression level of LC3B between the LC3B and age, gender, the location of the disease and mucosal lesion (affected area) (P> 0.05). However, there was no significant difference in the expression of LC3B between HP infection, dysplasia of gastric epithelium, chronic atrophic gastritis , The relative expression of intestinal metaplasia and LC3B was statistically significant (P <0.05). Conclusion: The high expression of LC3B mRNA and protein in autophagy is associated with HP infection and peptic ulcer with gastric atypical hyperplasia.