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目的:探讨急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)的免疫表型特点。方法:运用流式细胞术及特异性单克隆抗体CD2,CD3,CD4,CD5,CD7,CD8,CD10,CD13,CD19,CD20,CD22,CD33,CD34,CD38,CD45,CD56,CD117,HLA-DR,sIg M,cyIg,cyCD79a,MPO和TdT对58例形态学诊断ALL患者进行免疫表型检测。结果:通过CD3、CD19、CD33和MPO的表达情况分出T-ALL 8例(13.79%),B-ALL 49例(84.48%),混合细胞白血病1例(1.72%)。B-ALL进一步根据CD10,CD20,cyIg,sIg M的表达情况分为早前B型5例(8.62%),普通B型34例(58.62%),前B型9例(15.52%),成熟B型1例(1.72%)。B-ALL异常细胞存在抗原跨系列表达、跨阶段表达、缺失表达。儿童ALL免疫分型及白血病相关免疫表型与成人ALL差异无统计学意义。结论:ALL的异常骨髓细胞有明显本系列特征、原始细胞标记和跨系表达,在此基础上应用多参数流式细胞仪可有效检测微小残留病变。
Objective: To investigate the immunophenotypic characteristics of acute lymphoblastic leukemia (ALL). METHODS: Flow cytometry and specific monoclonal antibodies CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD13, CD19, CD20, CD22, CD33, CD34, CD38, CD45, CD56, CD117, , sIg M, cyIg, cyCD79a, MPO and TdT were detected in 58 cases with morphologic diagnosis of ALL. Results: 8 cases (13.79%) of T-ALL, 49 cases (84.48%) of B-ALL and 1 case of mixed cell leukemia (1.72%) were differentiated by the expression of CD3, CD19, CD33 and MPO. According to the expression of CD10, CD20, cyIg and sIg M, B-ALL was further divided into 5 cases (8.62%) of previous type B, 34 cases (58.62%) of common type B and 9 (15.52% B type in 1 case (1.72%). B-ALL abnormal cells exist across the series of antigen expression, cross-stage expression, deletion of expression. Child ALL immunophenotype and leukemia-related immunophenotype and adult ALL no significant difference. CONCLUSIONS: Allogeneic myeloid cells in ALL have obvious characteristics of this series, original cell markers and cross-lineage expression. On the basis of this, multi-parameter flow cytometry can effectively detect minimal residual disease.