论文部分内容阅读
利用含有12800个基因全长或部分序列的基因芯片研究结核分枝杆菌无毒株入侵导致的人巨噬细胞U937全基因组在转录水平的表达谱变化。结果表明其中473个基因(3.7％)差异表达,表达上调的基因25个(5.3％),上调超过3倍的包括丝氨酸/苏氨酸蛋白质激酶,可溶性结合半乳糖苷的凝集素,蛋白质酪氨酸磷酸酶delta,DDR受体酪氨酸激酶等的编码基因,其余基因(94.7％)表达下调。表达下调的基因中,表达仅为原来1/3以下的25个,其中syndecan结合蛋白的表达下调12.5倍.芯片研究结果与结核分枝杆菌一般抑制宿主细胞免疫应答的趋势相符。这473个基因中的376个基因在GenBank中有记录,另外97个是新基因。生物信息学分析提示差异表达基因编码产物与细胞内信号传导、细胞因子反应、细胞骨架重建、细胞凋亡、铁代谢、电子传递、线粒体功能和离子通道等有关。这些差异表达基因中,有17个是文献报道过的,而且都印证了本文的结果。RT-PCR和Northern杂交实验确证了表达谱芯片研究结果。通过DNA芯片研究全局表达谱变化,揭示了细菌入侵早期导致的巨噬细胞表达变化,为深入研究结核分枝杆菌-宿主相互作用提供了基础数据和探索方向。
The full-length or partial sequence of 12800 genes were used to study the transcriptional changes of the whole genome of human macrophage U937 caused by invading Mycobacterium tuberculosis non-virulent strains. The results showed that 473 genes (3.7%) were differentially expressed, 25 (5.3%) up-regulated genes were upregulated more than 3 times, which included serine / threonine protein kinase, soluble lectin binding galactoside, tyrosine Acid phosphatase delta, DDR receptor tyrosine kinase and other genes, the remaining genes (94.7%) were down-regulated. Among the down-regulated genes, only 25 were down-regulated from the previous one-third, and the expression of syndecan-binding protein was down-regulated by 12.5-fold.The results of the chip study were in agreement with the general tendency of Mycobacterium tuberculosis to suppress the host cell immune response. Of these 473 genes, 376 genes were recorded in GenBank and 97 were new genes. Bioinformatics analysis suggested that the products of differentially expressed genes were related to intracellular signal transduction, cytokine responses, cytoskeleton remodeling, apoptosis, iron metabolism, electron transport, mitochondrial function and ion channels. Of these differentially expressed genes, 17 were reported in the literature, and both confirmed the results of this article. RT-PCR and Northern hybridization experiments confirmed the results of the profiling microarray. The change of the global expression profiles by DNA chips revealed the changes of macrophages induced by bacterial invasion in the early stage, which provided the basic data and exploration direction for further study on the interaction between Mycobacterium tuberculosis and host.