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[目的]观察开心散对快速老化痴呆小鼠SAMP8炎症因子及β-APP影响。[方法]使用随机平行对照方法,将40只SAMP8小鼠按随机数字标法分为4组,10只/组,模型对照组、开心散组(高、低剂量组)、艾地苯醌组。正常对照组10只SAMR1小鼠。采用ELISA双抗体夹心法测定干预后脑组织及血清TNF-α、IL-8和β-APP含量。[结果]与正常对照组比较,模型组小鼠脑组织TNF-α、IL-8和β-APP含量明显升高(P<0.01),血清TNF-α、IL-8和β-APP浓度明显降低(P<0.01);与模型组比较,开心散低剂量组、开心散高剂量组小鼠脑组织TNF-α、IL-8和β-APP含量明显降低(P<0.01),血清TNF-α、IL-8和β-APP浓度明显升高(P<0.01)。[结论]开心散可减少脑组织的β-APP含量,降低炎症因子TNF-α、IL-8水平。
[Objective] To observe the effect of Kaixin powder on inflammatory factors and β-APP in SAMP8 mice with rapid aging dementia. [Methods] Forty SAMP8 mice were randomly divided into four groups according to a randomized parallel method: control group, model control group, Kaixin San (high and low dose groups), idebenone group . Normal control group 10 SAMR1 mice. The levels of TNF-α, IL-8 and β-APP in brain tissue and serum were detected by ELISA double antibody sandwich method. [Results] Compared with the normal control group, the levels of TNF-α, IL-8 and β-APP in the model group were significantly increased (P <0.01) and the concentrations of TNF-α, IL-8 and β- (P <0.01). Compared with the model group, the content of TNF-α, IL-8 and β-APP in the Kaixintosan low dosage group and Kaixin powder high dose group decreased significantly α, IL-8 and β-APP concentrations were significantly increased (P <0.01). [Conclusion] Kaixin powder can reduce the content of β-APP in brain tissue and decrease the levels of inflammatory cytokines TNF-α and IL-8.