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目的:探讨基质金属蛋白酶-9(MMP-9)抑制剂-强力霉素对脑出血大鼠神经再生的影响。方法:采用自体血注射法制作大鼠脑出血模型,随机分为对照组、模型组、干预组(脑出血后第1～7 d给予强力霉素30 mg/kg,1次/d,灌胃),第14 d处死动物并取脑,用免疫组化方法测定脑出血后血肿周围侧脑室嘴侧的室下区(SVZ)双皮质素(DCX)及MMP-9的表达。结果:与对照组(灰度值:MMP-9:116.87±5.13;DCX:121.34±2.76)相比,脑出血组大鼠在脑出血后第14 d时MMP9、DCX表达增强(灰度值:MMP-9:102.80±2.42,P<0.05;DCX:110.58±2.29,P<0.05)。干预组较脑出血组MMP-9表达减弱(灰度值:MMP-9:104.24±3.70,P<0.05),DCX表达增强(灰度值:DCX:106.50±3.12,P<0.01)。结论:脑出血急性期给予基质金属蛋白酶-9抑制剂强力霉素可以促进神经再生。
Objective: To investigate the effect of matrix metalloproteinase-9 (MMP-9) inhibitor, doxycycline, on the neural regeneration in rats with intracerebral hemorrhage. Methods: The model of intracerebral hemorrhage was established by autologous blood injection in rats. The rats were randomly divided into control group, model group and intervention group (Doxycycline 30 mg / kg, ). The animals were sacrificed on the 14th day and the brain was sacrificed. The expression of corticosterone (DCX) and MMP-9 in the subventricular zone (SVZ) of the periventricular area around the hematoma after intracerebral hemorrhage was determined by immunohistochemistry. Results: Compared with the control group (gray value: MMP-9: 116.87 ± 5.13; DCX: 121.34 ± 2.76), the expression of MMP9 and DCX increased on the 14th day after intracerebral hemorrhage in ICH rats (gray value: MMP-9: 102.80 ± 2.42, P <0.05; DCX: 110.58 ± 2.29, P <0.05). The expression of MMP-9 in the intervention group was weaker than that in the ICH group (gray value: MMP-9: 104.24 ± 3.70, P <0.05), and the expression of DCX was enhanced (gray value: DCX: 106.50 ± 3.12, P <0.01). Conclusion: The acute phase of cerebral hemorrhage given matrix metalloproteinase-9 inhibitor doxycycline can promote nerve regeneration.