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目的观察维生素C对三氧化二砷(AS2O3)抑制上皮性卵巢癌细胞株的化疗增敏效应并探讨其凋亡蛋白相关机制。方法以上皮性卵巢癌细胞株OVCAR-3为研究对象,以噻唑蓝(MTT)药敏试验、流式细胞仪以及蛋白质印迹法检测维生素C对AS2O3化疗的增敏效应及其机制。结果 AS2O3和Vitamin C单药对上皮性卵巢癌细胞株均具有剂量依赖性的生长抑制作用,IC50值分别为4.72μmol/L和21.85μmol/L。AS2O3联合Vitamin C的实验中,OVCAR-3细胞株的增殖抑制率均大于同浓度AS2O3单药的抑制率,P值分别为0.001和0.012;两组联合用药的q值分别为2.71和1.63,均大于1.15,提示两药联合具有协同抑制效应。流式细胞检测显示,AS2O3联合Vitamin C后S期阻滞效应明显,凋亡率增加。蛋白质印记法结果显示,两药联合作用后,Bcl-2表达明显下调,Bax表达明显上调。结论在上皮性卵巢癌细胞株,一定浓度范围的Vitamin C对AS2O3具有较好的化疗增敏效应,AS2O3和VitaminC的联合应用具有进一步研究的价值。
Objective To observe the sensitizing effect of vitamin C to chemotherapeutic effect of arsenic trioxide (As2O3) on epithelial ovarian cancer cell lines and to explore its mechanism of apoptosis protein. Methods The epithelial ovarian cancer cell line OVCAR-3 was used as the research object. The sensitizing effect of vitamin C to AS2O3 chemotherapy and its mechanism were determined by MTT susceptibility test, flow cytometry and Western blotting. Results Both AS2O3 and Vitamin C alone had a dose-dependent inhibitory effect on epithelial ovarian cancer cells with IC50 values of 4.72μmol / L and 21.85μmol / L, respectively. The inhibition rates of OVCAR-3 cell line were both higher than those of AS2O3 with the same concentrations of P2O3 (P = 0.001 and 0.012, respectively). The q values of the two groups were 2.71 and 1.63 Greater than 1.15, suggesting that the two drugs have a synergistic inhibitory effect. Flow cytometry showed that AS2O3 combined with Vitamin C had obvious S phase arrest effect and increased apoptosis rate. Western blot results showed that the combination of the two drugs, Bcl-2 was significantly down-regulated, Bax was significantly up-regulated. Conclusion In epithelial ovarian cancer cell lines, Vitamin C at a certain concentration range has better chemosensitizing effect on AS2O3. The combination of AS2O3 and Vitamin C has the value of further research.