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利用苯并噻二唑BTH处理菌核病抗性不同的花椰菜品种幼苗,采用营养生长期活体叶片菌丝块接种鉴定法评价菌核病抗性诱导效果,结果表明经BTH处理的植株菌核病病情指数明显下降,对感病品种和抗病品种的诱抗效果分别达到81.5%和63.8%。对于花椰菜重要的防御酶活性变化研究结果表明,BTH诱导处理的花椰菜植株过氧化物酶(POD)、抗坏血酸酶(SOD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)的活性均有所提高。同时病程相关蛋白几丁质酶和β-1,3-葡聚糖酶的活性也增加。利用半定量RT-PCR方法检测防御反应基因表达,结果表明BTH诱导首先激发了植株PR-1等基因参与的水杨酸信号传导防御反应途径的发生,同时PDF1.2基因的上调表达说明BTH诱导也影响了茉莉酸信号传导途径。
Using benzothiadiazole BTH to treat sclerotinia seedlings with different sclerotinia resistance, the inoculation method was used to evaluate the resistance of Sclerotinia sclerotiorum in vegetative growth stage. The results showed that the sclerotinia sclerotiorum The disease index decreased significantly, and the induced resistance to susceptible and resistant cultivars reached 81.5% and 63.8% respectively. The results showed that the activities of POD, CAT, CAT and Bcl - 2 in Cauliflower induced by BTH were higher than those in Cauliflower And polyphenol oxidase (PPO) activity have increased. At the same time, the activity of chitinase and β-1, 3-glucanase related to disease course also increased. The expression of defense response genes was detected by semi-quantitative RT-PCR. The results showed that BTH induced the salicylic acid signal transduction defense pathway involved in the PR-1 gene expression. The up-regulated expression of PDF1.2 gene indicated that BTH induction It also affects the jasmonic acid signaling pathway.