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目的 :克隆日本血吸虫大陆株副肌球蛋白部分基因。方法 :用 PCR方法根据已发表日本血吸虫菲律宾株副肌球蛋白基因部分核苷酸序列扩增大陆株副肌球蛋白基因。结果 :获得一日本血吸虫大陆株 72 0 bp基因克隆。结论 :经核苷酸序列分析证实 ,本克隆基因与菲律宾株基因核苷酸同源性为 99.0 3%。
Objective: To clone partial myosin partial gene of Schistosoma japonicum. Methods: The paramyosin gene was amplified by polymerase chain reaction (PCR) from the nucleotide sequence of the paramyosin gene of S. paratyphoid strain. Results: The 72 0 bp gene of Schistosoma japonicum was cloned. Conclusion: Nucleotide sequence analysis confirmed that the nucleotide sequence of this clone and the Philippines strain was 99.0 3%.