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根据番茄溃疡病菌ITS序列,设计并合成了PCR-DHPLC检测引物,对番茄溃疡病菌及其他病菌共10个标准菌株进行了PCR-DHPLC检测。结果表明,番茄溃疡病菌的PCR-DHPLC检测图谱出现了特异性吸收峰,而其他病菌均未在相同洗脱时间出现吸收峰,说明这种方法具有检测番茄溃疡病菌的特异性。灵敏度实验结果表明,PCR-DHPLC体系与PCR-琼脂糖凝胶电泳体系的检测灵敏度一致。研究表明,PCR-DHPLC方法是一种特异、灵敏、快速的番茄溃疡病菌检测方法。
PCR-DHPLC primers were designed and synthesized based on the ITS sequence of tomato canker. PCR-DHPLC was performed on 10 standard strains of tomato canker and other pathogens. The results showed that there was a specific absorption peak of PCR-DHPLC in tomato canker, while none of the other pathogens showed the same absorption peak at the same elution time, indicating that this method has the specificity of detecting tomato canker. Sensitivity experimental results show that, PCR-DHPLC system and PCR-agarose gel electrophoresis system detection sensitivity. Studies have shown that, PCR-DHPLC method is a specific, sensitive and rapid tomato canker detection method.