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为进一步阐明脑缺血后细胞凋亡的发生及其调控的分子机理,本实验利用大鼠大脑中动脉(MCA)局灶性缺血模型,采用免疫组化、原位杂交、反转录PCR等技术,探讨大脑缺血再灌注损伤下,神经细胞死亡过程中细胞凋亡的现象。材料与方法用动脉内置线阻断法建立大鼠MCA...
In order to further elucidate the molecular mechanism of apoptosis after cerebral ischemia, we used the model of focal middle cerebral artery (MCA) in rats to establish a model of focal ischemia by immunohistochemistry, in situ hybridization and reverse transcription PCR And other techniques to explore the phenomenon of apoptosis in the process of neuronal cell death under cerebral ischemia-reperfusion injury. Materials and Methods Rat MCA was established by intra-arterial line blockade