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以大豆为实验材料,研究了波长为532 nm的Nd:YAG倍频激光不同功率密度和不同照射时间对大豆发芽率、幼苗平均株高、幼苗子叶及叶片异黄酮含量的影响。从光合效率和植物异黄酮合成代谢起始酶苯丙氨酸转氨酶(PAL)活性两方面探讨激光预处理大豆种子提高其幼苗异黄酮含量的机理。结果表明,大豆种子经激光预处理后,其幼苗可溶性糖、蔗糖、淀粉等光合同化产物及异黄酮合成代谢起始酶PAL活性均得到提高,其中以15 mW/mm2激光预处理大豆种子5 min的效果最佳。幼苗光合效率得到了提高,为异黄酮的大量积累提供了基础。
The effects of different power density and different irradiation time of 532 nm Nd: YAG frequency laser on germination rate, seedling average plant height, seedling cotyledon and leaf isoflavone content were studied using soybean as experimental material. The mechanisms of laser pretreatment of soybean seed to increase its seedling isoflavone content were studied from photosynthetic efficiency and PAL phenylase activity of plant isoflavone anabolism. The results showed that the photosynthetic assimilation products of soluble sugar, sucrose and starch, and the PAL activity of isoflavone anabolic enzymes in soybean seed were enhanced after laser pretreatment. The seed of soybean seed was pretreated with 15 mW / mm2 laser min the best effect. Seedling photosynthetic efficiency has been increased, providing a basis for the large-scale accumulation of isoflavones.