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为研究缺血预处理对缺血再灌注后兔腰髓组织中HSPs 70mRNA表达的影响 ,探讨缺血预处理对脊髓缺血再灌注损伤保护作用的机制 ,经股动脉向腹主动脉内置入 4FSwan Ganz导管 ,导管气囊置于左肾动脉开口远端 0 5～ 1 0cm ,向气囊内注气建立脊髓缺血模型。将实验兔分为假手术组、对照组和预处理组 ,应用RT PCR方法对 3组兔缺血再灌注后不同时间点脊髓组织中HSPs 70mRNA表达进行观察。结果发现 ,假手术组兔的脊髓组织没有HSPs 70mRNA的阳性表达 ,缺血再灌注后 ,预处理组兔脊髓组织的HSPs 70mR NA表达较对照组明显增强(P <0 0 1) ,表达时间也显著延长。提示缺血预处理可以明显增强缺血后脊髓组织中HSPs 70mRNA的表达 ,延长HSPs 70mRNA的表达时间 ,并可能通过此机制对缺血脊髓产生保护作用
To investigate the effect of ischemic preconditioning on the expression of HSP70mRNA in rabbit lumbar spinal cord after ischemia-reperfusion, to explore the mechanism of ischemic preconditioning on the protection of spinal cord ischemia-reperfusion injury, the transposition of 4FSwan into the abdominal aorta via the femoral artery Ganz catheter, catheter balloon placed in the distal left renal artery opening 0 5 ~ 1 0cm, inflatable balloon to establish spinal cord ischemia model. The rabbits were divided into sham operation group, control group and preconditioning group. RT-PCR method was used to observe the expression of HSP70 70mRNA in spinal cord at different time points after ischemia-reperfusion. The results showed that there was no positive expression of HSP70mRNA in the spinal cord of sham operation group. Compared with the control group, the expression of HSP70mRNA in spinal cord of preconditioning group was significantly increased after ischemia / reperfusion (P <0.01) Significantly longer. These results suggest that ischemic preconditioning can significantly increase the expression of HSP70mRNA in ischemic spinal cord tissue and prolong the expression of HSP70mRNA, which may have a protective effect on ischemic spinal cord