扩增拟南芥(Arabidopsis thaliana)螯合肽合成酶(AtPCS1)全长基因;构建AtPCS1植物表达载体pBⅠ121-AtPCS1,进一步转化农杆菌EHA105;利用转化的农杆菌EHA105侵染甘农一号苜蓿(Medicago sativa)叶片,经过80~100 d的筛选与培养,获得57株再生转基因植株。随机抽取其中9株进行PCR检测,其中6株为阳性。初步结果表明,AtPCS1基因已整合到苜蓿基因组中。 (AtPCS1) was amplified from Arabidopsis thaliana. The AtPCS1 plant expression vector pBⅠ121-AtPCS1 was constructed and transformed into Agrobacterium tumefaciens EHA105. Agrobacterium tumefaciens strain EHA105 was used to infect Gannong No.1 Medicago sativa Medicago sativa leaves. After 80-100 days of selection and culture, 57 regenerated transgenic plants were obtained. Nine of them were randomly selected for PCR test, of which 6 were positive. Preliminary results show that the AtPCS1 gene has been integrated into the alfalfa genome.