本研究采用Dig-HBV-DNA探针检测了57例新生儿及其父、母血液、羊水、初乳和脐血6个标本及HBV标志物的检测、HBV-DNA检出率母血47.37％,父血42.11％,脐血40.35％,羊水7.02％,初乳22.81％,新生儿血为49.2％。14例抗-HBs阳性产妇,其自身HBV-DNA检出达42.86％,抗-HBs可通过胎盘传给新生儿,新生儿血中抗-HBs阳生率42.86％,HBV-NDA检出率为50％。同时亦观察了父母HBV感染对新生儿的影响,两者差异显著。 In this study, sixty samples from 57 newborns and their parents, maternal blood, amniotic fluid, colostrum and umbilical cord blood were detected by using Dig-HBV-DNA probe. The detection rate of HBV-DNA was 47.37% 42.11% of father’s blood, 40.35% of umbilical cord blood, 7.02% of amniotic fluid, 22.81% of colostrum and 49.2% of newborn’s blood. 14 cases of anti-HBs positive mothers, the HBV-DNA detection of its own up to 42.86%, anti-HBs can be passed to the newborn placenta, neonatal anti-HBs positive rate of 42.86%, HBV-NDA detection rate 50%. At the same time also observed the impact of parental HBV infection on the newborn, the difference was significant.