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水稻(Oryza sativa)萌发耐淹性受到复杂的网络调控,其分子机制不同于苗期耐淹性的相关机制,萌发耐淹性的强弱影响着直播稻的成苗率。通过对256份水稻核心种质的萌发耐淹性评估,发现粳稻和籼稻之间的萌发耐淹性差异并不显著,都存在广泛的遗传变异。利用以粳稻R0380为供体亲本,籼稻RP2334为轮回亲本的170个高代回交自交系构建含146个分子标记的连锁图谱,以低氧胚芽鞘长度为性状指标,通过复合区间作图法检测到影响萌发耐淹性的4个QTLs(quantitative trait loci),分别定位于第2(2个)、3(1个)和8号(1个)染色体。贡献率最大的QTL为qGS2.2,其值为17.34%,增效等位基因来自轮回亲本籼稻RP2334;其余3个QTLs的增效等位基因均来自供体亲本粳稻R0380,贡献率分别为12.86%、9.37%和14.60%。
The flooding resistance of Oryza sativa is complicated by network regulation. The molecular mechanism of Oryza sativa is different from the mechanism of flooding resistance at seedling stage, and the seed germination rate of direct-seeded rice is influenced by the seedling flooding resistance. By evaluating the flooding resistance of 256 core collection of rice germplasm, it was found that there was no significant difference in the germination flooding resistance between japonica and indica, and there was a wide range of genetic variation. A linkage map of 146 molecular markers was constructed by using 170 high-generation backcross inbred lines with japonica R0380 as donor parent and indica rice RP2334 as reincarnation parent. With the hypoxia coleoptile length as trait marker, Four QTLs (quantitative trait loci) affecting the seedling flooding resistance were detected, which were located on chromosomes 2 (2), 3 (1) and 8 (1) respectively. The QTL with the largest contribution rate was qGS2.2 with a value of 17.34%, and the synergistic allele was from the recurrent parent RP2334. The other three QTLs with synergistic alleles were from the donor parent R0380 with the contribution rates of 12.86 %, 9.37% and 14.60%.