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目的:从肾气丸“阴中求阳”配伍对肾阳虚模型大鼠HPG轴的影响,初步探讨其“阴中求阳”的配伍机理。方法:将SD雄性大鼠随机分为正常对照组、模型组、补阳组、补阴组、阴阳双补组,观察大鼠一般活动。大腿内侧肌肉丰厚处注射氢化可的松12天制造肾阳虚模型。成模后各组灌胃治疗20天,第21天上午8时取血,采用酶联免疫法(ELISA)检测血清PRL、FSH、LH、E2、T水平。取睾丸、附睾、精囊、前列腺称重,测睾丸、附睾、精囊、前列腺重量指数。结果:阴阳双补组能增加肾阳虚模型大鼠体重,升高睾丸、附睾、精囊、前列腺重量指数,改变血清PRL、FSH、LH、E2、T水平,其作用明显优于补阳组或补阴组。补阳组与补阴组比较,在增加模型大鼠体重方面,补阳组效果优于补阴组;在升高睾丸、附睾、精囊、前列腺重量指数方面,补阴组效果略微优于补阳组,但二者之间差异不明显。各治疗组中,补阳组效果仅在影响PRL、LH水平方面有统计学意义。结论:针对肾阳虚证,单纯补阳、补阴效果不明显,阴阳双补可提高疗效,初步阐释了肾气丸“阴中求阳”的配伍理论。
OBJECTIVE: To study the effect of compatibility of Shenqi Pill “yinzhongyang ” on HPG axis in rats with kidney-yang deficiency and to explore the compatibility mechanism of “Qi Yin in Yang”. Methods: Male Sprague-Dawley rats were randomly divided into normal control group, model group, BuYang group, BuYinYu group and Yin-Yang combined group. The general activities of rats were observed. Inner thigh muscular injection of hydrocortisone 12 days to create kidney yang deficiency model. The rats in each group were given gavage for 20 days after operation and blood was collected on the 21st day at 8 am. Serum levels of PRL, FSH, LH, E2 and T were detected by enzyme-linked immunosorbent assay (ELISA). Testis, epididymis, seminal vesicle, prostate weighing, testicular, epididymis, seminal vesicle, prostate weight index. Results: Yin and Yang Shuibu group can increase body weight, increase testis, epididymis, seminal vesicle, prostate weight index, and change the levels of serum PRL, FSH, LH, E2, T in kidney-yang deficiency rats Yin group. Buyang group and Buyin group, increase the body weight of rats in the model group, Buyang group is better than Buyin group; increase testis, epididymis, seminal vesicle, prostate weight index, Yin Buyin effect is slightly better than Yang Group, but the difference between the two is not obvious. In each treatment group, the effect of Buyang group only had statistical significance in influencing PRL and LH levels. Conclusion: For the syndrome of deficiency of kidney-yang, simple yang and yin-yang effect is not obvious, yin and yang shu-bian can improve the curative effect, initially explained the compatibility theory of yin-qi pill.