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目的 探讨颅内肿瘤脑脊液端粒酶活性的表达 ,评价其作为肿瘤标记物及临床意义。方法 取 19例颅内肿瘤和 5例脑外伤患者脑脊液标本 ,应用改良的银染端粒重复序列扩增法 (TRAP)进行端粒酶活性检测。结果 19例颅内肿瘤脑脊液标本中 3例表现出端粒酶活性 ,阳性率 15 .8% ( 3 / 19) ,3例阳性者分别为室管膜瘤、肺癌脑转移和复发胶质瘤。 5例脑外伤患者脑脊液标本无端粒酶活性表达。结论 脑脊液中端粒酶活性检测对评估颅内肿瘤生物学特性和临床意义具有重要参考价值。
Objective To investigate the expression of telomerase activity in cerebrospinal fluid of intracranial tumors and evaluate its clinical significance as a tumor marker. Methods Cerebrospinal fluid was collected from 19 patients with intracranial tumors and 5 patients with traumatic brain injury. Telomerase activity was detected using a modified silver-labeled telomeric repeat amplification protocol (TRAP). Results Three of the 19 CSF samples showed telomerase activity, the positive rate was 15.8% (3/19). The positive results were ependymoma, brain metastasis of lung cancer and recurrent glioma. Cerebrospinal fluid specimens of 5 patients with traumatic brain injury showed no telomerase activity. Conclusion Detection of telomerase activity in cerebrospinal fluid plays an important reference value in evaluating the biological characteristics and clinical significance of intracranial tumors.