论文部分内容阅读
水稻类病变突变体lmi(lesion mimic initiation)是从γ射线诱变的籼稻品种中籼3037的后代中发现的,属于起始型的类病变突变体.无菌培养、台盼蓝染色及遮光实验表明,该突变体受光照控制细胞自主性死亡.遗传分析表明,该突变性状由一对隐性基因控制.利用lmi和93-11杂交的F2群体对lmi基因进行初步遗传定位,发现该基因定位于水稻第8号染色体着丝粒附近的两个微卫星分子标记RM547和RM331之间,与两者遗传距离分别为1.2和3.2 cM.进一步利用这两个标记之间发展的CAPS标记C4135-8,C4135-9及C4135-10对lmi基因进行精细的遗传定位,结果表明,lmi基因与标记C4135-10共分离.这一结果为克隆lmi基因奠定了基础.
Rice lesion mutant lmi (lesion mimic initiation) was found in the offspring of indica rice variety Zhongxian 3037, belonging to the initial type of disease-like mutant. Sterile culture, trypan blue staining and shading experiments The results showed that the mutant was autonomously controlled by light and the genetic control indicated that the mutant was controlled by a pair of recessive genes.The genetic mapping of lmi gene was carried out by using F2 population of lmi and 93-11 hybrids The genetic distance between the two microsatellite markers RM547 and RM331 near the centromere on chromosome 8 of rice was 1.2 and 3.2 cM, respectively, and the CAPS marker C4135-8 developed between these two markers , C4135-9 and C4135-10. The results showed that the lmi gene was co-segregated with the marker C4135-10, which laid the foundation for the cloning of the lmi gene.