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本文介绍用反相ＨＰＬＣ法同时测定三环类抗抑郁药：多虑平、丙咪嗪、阿米替林、氯丙咪嗪的血药浓度。流动相为甲醇－水－四甲基乙二胺（７０∶３０∶１），冰醋酸调ｐＨ至６．４。选用安定为内标，体液样本在碱性条件下，用无水乙醚提取，氮气流挥干后用流动相溶解进样。线性范围为１０～６００ｎｇ／ｍｌ，最低检测浓度为５～１０ｎｇ／ｍｌ，回收率为９３．３３％～９７．７９％，相对标准偏差为１．００％～１．８２％，并用该方法对患者血清进行了测定。
This article describes the simultaneous determination of tricyclic antidepressants: doxepin, imipramine, amitriptyline, clomipramine by reverse phase HPLC method. The mobile phase was methanol-water-tetramethylethylenediamine (70: 30: 1), and the pH was adjusted to 6.4 with glacial acetic acid. Selection of stability as an internal standard, body fluid samples in alkaline conditions, extracted with anhydrous ether, evaporated to dryness after the flow of nitrogen dissolved sample. The linear range was 10 ~ 600ng / ml, the lowest detection concentration was 5 ~ 10ng / ml, the recovery was 93.33% ~ 97.79% and the relative standard deviation was 1.00% ~ 1.82% Patient serum was measured.