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目的探讨妊娠中晚期羊水细胞培养和染色体核型分析在临床应用中的可行性。方法收集近3年来在我院行产前诊断、孕23周以后的孕妇羊水标本共124例。从羊水细胞接种、培养和收获方法进行改良,最后行染色体核型分析。结果124例孕中晚期羊水细胞培养全部一次性成功,成功率达100%;124例患者中共检出异常染色体核型8例(6.5%);检出染色体多态性11例(8.9%)。结论改良妊娠中晚期羊水染色体培养成功率高,染色体分裂相较多,效果较好,可满足临床妊娠中晚期孕妇产前诊断的需求。
Objective To investigate the feasibility of amniotic fluid cell culture and chromosome karyotype analysis in the second trimester of pregnancy. Methods Collection of prenatal diagnosis in our hospital in the past 3 years, 124 cases of pregnant women with amniotic fluid after 23 weeks pregnant. From amniotic fluid cells inoculation, culture and harvest methods were improved, the final line of chromosome karyotype analysis. Results 124 cases of middle and late pregnancy amniotic fluid cell culture all with one success, the success rate was 100%; 124 cases of abnormal chromosome karyotype detected in 8 cases (6.5%); detected chromosome polymorphism in 11 cases (8.9%). Conclusion The improved amniotic fluid chromosome in late pregnancy has a high success rate, more chromosomes and better results, which can meet the needs of prenatal diagnosis of pregnant women in the second and third trimester of pregnancy.