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AIM: To investigate the impact of titanium dioxide nanoparticles(Ti O2NPs) on embryonic development and retinal neurogenesis.METHODS: The agglomeration and sedimentation of Ti O2 NPs solutions at different dilutions were observed,and the ultraviolet-visible spectra of their supernatants were measured. Zebrafish embryos were experimentally exposed to Ti O2 NPs until 72 h postfertilization(hpf). The retinal neurogenesis and distribution of the microglia were analyzed by immunohistochemistry and whole mount in situ hybridization.RESULTS: The 1 mg/L was determined to be an appropriate exposure dose. Embryos exposed to Ti O2 NPs had a normal phenotype. The neurogenesis was initiated on time, and ganglion cells, cones and rods were well differentiated at 72 hpf. The expression of fms m RNA and the 4C4 antibody, which were specific to microglia in the central nervous system(CNS), closely resembled their endogenous profile.CONCLUSION: These data demonstrate that short-term exposure to Ti O2 NPs at a low dose does not lead to delayed embryonic development or retinal neurotoxicity.
AIM: To investigate the impact of titanium dioxide nanoparticles (Ti O2 NPPs) on embryonic development and retinal neurogenesis. METHODS: The agglomeration and sedimentation of Ti O2 NPs solutions at different dilutions were observed, and the ultraviolet-visible spectra of their supernatants were measured. The retinal neurogenesis and distribution of the microglia were analyzed by immunohistochemistry and whole mount in situ hybridization .RESULTS: The 1 mg / L was determined to be appropriate for exposure to Zebrafish embryos were experimentally exposed to Ti O2 NPs until 72 h postfertilization (hpf) The expression of fms m RNA and the 4C4 antibody, which were specific to microglia in the central nervous system (CNS), closely resembled their endogenous profile. CONCLUSION: These data demonstrate that short-term exposure to Ti O2 NP s at a low dose does not lead to delayed embryonic development or retinal neurotoxicity.