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目的 :观察丙泊酚、咪达唑仑和硫喷妥钠对鼠局灶脑缺血再灌注后缺血部位氨基酸堆积的影响。方法 :雄性SD大鼠 ,大脑中动脉内线栓法诱发3h局灶脑缺血和 3h再灌注。于再灌注前分别给予丙泊酚、咪达唑仑和硫喷妥钠 5 0mg·kg- 1,ip。再灌注 3h后用高效液相色谱法 (HPLC)测脑组织氨基酸浓度。结果 :丙泊酚和咪达唑仑减少缺血组织内门冬氨酸和谷氨酸的堆积。丙泊酚增强 ,咪达唑仑减小γ -氨基丁酸的堆积。硫喷妥钠无明显作用。结论 :丙泊酚和咪达唑仑可能通过加快再灌注时兴奋性氨基酸的清除而产生脑保护效应。
Objective: To observe the effects of propofol, midazolam and thiopental on the accumulation of amino acids in ischemic area after focal cerebral ischemia-reperfusion in rats. Methods: Male SD rats and middle cerebral artery occlusion were induced by 3h focal cerebral ischemia and 3h reperfusion. Before reperfusion, propofol, midazolam and thiopental 50 mg · kg-1, ip respectively. After 3h of reperfusion, the brain tissue amino acid concentration was measured by high performance liquid chromatography (HPLC). Results: Propofol and midazolam reduced accumulation of aspartate and glutamate in ischemic tissue. Propofol enhanced, midazolam reduced γ - aminobutyric acid accumulation. Thiopental no significant effect. CONCLUSION: Propofol and midazolam may exert a neuroprotective effect by accelerating the clearance of excitatory amino acids during reperfusion.