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目的探讨环氧化酶-2(COX-2)在胃癌组织中的表达及其与肿瘤血管生成的关系。方法应用免疫组织化学(免疫组化)法检测96例胃癌手术切除标本中COX-2的表达,采用抗CD34抗体标记微血管内皮细胞,计算微血管密度(MVD)。分析COX-2表达与MVD和胃癌主要临床病理特征的相关性。结果胃癌组织COX-2阳性表达率和MVD分别为80.2%和32.5±8.3,显著高于正常胃黏膜的13.3%和13.1±2.4;P<0.01。临床TNM分期中Ⅲ、Ⅳ期胃癌标本COX-2表达率与MVD为91.4%和34.9±8.7,显著高于Ⅰ和Ⅱ期胃癌标本(P<0.01)。伴有淋巴结转移的胃癌组织中COX-2表达率和MVD分别为87.9%和35.0±8.5,与无淋巴结转移病例相比,差异有统计学意义(P<0.05)。Spearman等级相关分析表明,COX-2表达与MVD呈显著正相关(γ=0.311,P<0.01)。结论COX-2表达在胃癌的肿瘤血管生成中起重要作用,COX-2及其诱导的血管生成与胃癌的浸润和转移密切相关。
Objective To investigate the expression of cyclooxygenase-2 (COX-2) in gastric carcinoma and its relationship with tumor angiogenesis. Methods The expression of COX-2 in 96 specimens of gastric cancer was detected by immunohistochemistry (IHC). Microvessel density (MVD) was calculated by labeling anti-CD34 antibody with microvessel endothelial cells. The correlation between the expression of COX-2 and the major clinicopathological features of MVD and gastric cancer was analyzed. Results The positive rates of COX-2 and MVD in gastric cancer tissues were 80.2% and 32.5 ± 8.3, respectively, which were significantly higher than those in normal gastric mucosa (13.3% and 13.1 ± 2.4; P <0.01). The expression rates of COX-2 and MVD in stage Ⅲ and Ⅳ gastric cancer specimens were 91.4% and 34.9 ± 8.7, respectively, which were significantly higher than those in stage Ⅰ and Ⅱ gastric cancer specimens (P <0.01). The expression of COX-2 and MVD in gastric cancer with lymph node metastasis were 87.9% and 35.0 ± 8.5, respectively, which were significantly different from those without lymph node metastasis (P <0.05). Spearman rank correlation analysis showed that COX-2 expression was positively correlated with MVD (γ = 0.311, P <0.01). Conclusions COX-2 expression plays an important role in tumor angiogenesis in gastric cancer. COX-2 and its angiogenesis are closely related to the infiltration and metastasis of gastric cancer.