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目的研究丹皮总苷（ＴＧＭ）体外对３类免疫细胞功能的影响。方法采用［３Ｈ］－ＴｄＲ参入法，检测Ｔ淋巴细胞增殖反应和分泌白介素２（ＩＬ－２）活性，Ｂ淋巴细胞增殖反应以及巨噬细胞产生ＩＬ－１。结果ＴＧＭ２～５０ｍｇ·Ｌ－１可明显促进刀豆蛋白Ａ诱导小鼠Ｔ淋巴细胞增殖反应和大鼠Ｔ淋巴细胞产生ＩＬ－２，ＴＧＭ还可促进脂多糖诱导Ｂ淋巴细胞增殖反应以及大鼠腹腔巨噬细胞产生ＩＬ－１，它们的浓度－效应曲线呈钟罩形。结论ＴＧＭ具有浓度依赖性双向免疫调节作用。
Objective To study the effect of total glucosides of trefoil (TGM) on the function of three types of immune cells in vitro. Methods [3H]-TdR incorporation assay was used to detect the proliferation of T lymphocytes and the secretion of interleukin-2 (IL-2) activity, proliferation of B lymphocytes, and production of IL-1 by macrophages. Results TGM2～50mg·L-1 could obviously promote the proliferation of mouse T lymphocytes induced by ConA and IL-2 in rat T lymphocytes. TGM could also promote the proliferation of B lymphocytes induced by lipopolysaccharide and rat abdominal cavity. Macrophages produce IL-1 and their concentration-effect curves are bell-shaped. Conclusion TGM has a concentration-dependent bidirectional immunomodulatory effect.