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目的:优化超临界流体(SFE)萃取白花蛇舌草挥发油工艺参数,并对萃取产物的化学成分进行GC-MS分析。方法:采用正交试验法,以出油率为衡量指标,考察超临界CO2萃取的影响因素(萃取压力、萃取温度、药材粒度),确定其较佳工艺水平,用GC-MS对萃取产物进行分离分析,应用图谱库检索结合KI指数确定各化合物结构,用色谱峰面积归一化法确定萃取产物中各成分的相对百分含量。结果:白花蛇舌草超临界萃取出油率的最佳工艺条件为压力25 MPa、温度50℃,药材粒度20～40目。萃取产物GC-MS结果显示,白花蛇舌草中除含高级脂肪酸及其酯类外,还含有大量的甾族化合物。结论:超临界流体萃取白花蛇舌草挥发油是稳定和可行的;利用GC-MS分析技术结合保留指数鉴定结构的方法准确、快速。
OBJECTIVE: To optimize the SFE extraction process parameters of volatile oil from Hedyotis diffusa Willd. The chemical constituents of the extract were analyzed by GC-MS. Methods: Using orthogonal test method, take the oil yield as the measurement index, investigate the influencing factors of supercritical CO2 extraction (extraction pressure, extraction temperature, medicine particle size), determine the better technology level, using GC-MS to extract the product Separation and analysis, the application of the combination of KI index library map to determine the structure of each compound, with the chromatographic peak area normalization method to determine the relative content of each component of the extracted product. Results: The optimum conditions of supercritical fluid extraction of Hedyotis diffusa were 25 MPa pressure, 50 ℃ temperature and 20-40 mesh size. The result of GC-MS showed that Hedyotis diffusa had a large amount of steroids besides the higher fatty acids and esters. Conclusion: The supercritical fluid extraction of Hedyotis diffusa volatile oil is stable and feasible; GC-MS analysis using retention index identification method is accurate and fast.