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衰老是一个主动的过程,包括细胞结构、新陈代谢、基因表达有序发生变化,对植物生存繁衍具有积极的意义,但早衰则对农业生产会产生重要影响,不利于经济性状的获得,研究早衰的分子机理具有重要的意义.利用甲基磺酸乙酯诱变恢复系缙恢10号获得了一个叶片早衰突变体,5叶期前叶片正常绿色,从6叶至剑叶每张叶片从叶尖到叶基部逐渐衰老,叶绿体膜结构破坏、光合色素含量和光合能力及可溶性蛋白含量显著下降,SOD酶活性异常.遗传分析显示该突变性状受一显性单基因控制,暂命名为psl3(presenescing leaf3).利用分子标记将PSL3基因定位于第7染色体标记c7sr1与ID10之间,物理距离为53.5kb,为该基因的图位克隆奠定了基础.
Aging is an active process, including cell structure, metabolism, orderly changes in gene expression, the survival and reproduction of plants has a positive meaning, but premature aging will have an important impact on agricultural production, is not conducive to the acquisition of economic traits, premature aging Molecular mechanism is of great significance.Using methyl ethyl sulfonate restorer restore line Jinhui 10 access to a leaf premature senility mutant, 5 leaves before the normal green leaves, leaves from the 6 leaves to each leaf blade from the tip The leaf senescence gradually progressed, the structure of chloroplast membrane was destroyed, photosynthetic pigment content, photosynthetic capacity and soluble protein content decreased significantly, and SOD activity was abnormal.Genetic analysis showed that this mutant trait was controlled by a dominant single gene and tentatively named psl3 (presenescing leaf3 ). The molecular marker was used to map the PSL3 gene between chromosome 7 c7sr1 and ID10 on the 7th chromosome. The physical distance was 53.5kb, which laid the foundation for the mapping of this gene.