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目的通过对结直肠癌(CRC)组织中多基因甲基化水平的检测,探讨多基因甲基化对CRC早期诊断的意义。方法选取25例CRC患者胃镜采集标本组织,通过甲基化特异性PCR(MSP)检测CRC和炎性组织中甲基化水平,分析其与CRC患者临床各指标的关系。结果在CRC患者中,APC、P16、MLH1、DCC甲基化率分别为52.0%(13/25)、32.0%(8/25)、44.0%(11/25)、60.0%(15/25)。炎性组织中仅有1例DCC甲基化异常表达,甲基化率为10.0%(1/10),甲基化阳性率显著高于炎性组织(P<0.05),4种基因甲基化联合检测诊断CRC的阳性率为92.0%(23/25),高于单个基因检测的甲基化阳性率,差异有统计学意义(P<0.01)。结论 APC、P16、MLH1、DCC基因在CRC患者中甲基化水平异常表达,联合检测APC、P16、MLH1、DCC可能作为CRC早期诊断的标志物。
Objective To investigate the significance of multi-gene methylation in the early diagnosis of CRC by detecting the multi-gene methylation level in colorectal cancer (CRC). Methods 25 cases of CRC patients were collected by gastroscopy. Methylation-specific PCR (MSP) was used to detect the methylation level in CRC and inflammatory tissues, and its relationship with clinical indicators of CRC were analyzed. Results The methylation rates of APC, P16, MLH1 and DCC in CRC patients were 52.0% (13/25), 32.0% (8/25), 44.0% (11/25) and 60.0% (15/25) . Only one case of DCC methylation was abnormally expressed in the inflammatory tissues, the methylation rate was 10.0% (1/10), the positive rate of methylation was significantly higher than that of the inflammatory tissues (P <0.05) The positive rate of combined detection of CRC was 92.0% (23/25), which was higher than the positive rate of methylation detected by single gene (P <0.01). Conclusions The methylation level of APC, P16, MLH1 and DCC genes is abnormally expressed in CRC patients. Combined detection of APC, P16, MLH1 and DCC may be used as a marker for early diagnosis of CRC.