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[Objective] The paper was to reveal the species abundance of associated bacterial community of Radopholus similis, and the diversity of its bacteria. [Methods] Through the construction of 16S rDNA cloning library of bacteria, the positive clone was carried out ARDRA analysis, and the phylogenetic tree was constructed. [Results] OUT (Operational Taxonomic Units) was classified according to sequences similarity of 97%, and the constructed 16S rDNA library of bacteria contained 13 OTUs, belonging to Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria, respectively. The dominant species was Betaproteobacteria, especially Burkholderia was the dominant bacterium. [Conclusions] The diversity of associated bacteria of R. similis was high, and these bacteria may have certain ecological significance to R. similis.
[Objective] The paper was to reveal the species abundance of associated bacterial community of Radopholus similis, and the diversity of its bacteria. [Methods] Through the construction of 16S rDNA cloning library of bacteria, the positive clone was carried out ARDRA analysis, and The phylogenetic tree was constructed. [Results] OUT (Operational Taxonomic Units) was classified according to sequences similarity of 97%, and the constructed 16S rDNA library of bacteria contained 13 OTUs, belonging to Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria, respectively. was Betaproteobacteria, especially Burkholderia was the dominant bacterium. [Conclusions] The diversity of associated bacteria of R. similis was high, and these bacteria may have an ecological significance to R. similis.