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The aim of this study is to elucidate the molecular and cellular mechanisms underlying the immunosuppressive effect of Sanchi extract(SE)via investigating the effects of SE on the activation and proliferation of murine lymphocytes and NO secretion by peritoneal macrophages in vitro.ConA was used to activate lymphocytes,and expression of CD69 on T cells and CFSE labeled cell division were detected by flow cytometry.Murine peritoneal macrophages were stimulated with LPS or lymphocytes culture supemate(LCS)and the concentration of NO was determined by Griess reagent assay.After 6 h of culture,SE ranging from 50 to 100μg/ml downregulated CD69 expression on ConA-activated T cells,while SE ranging from 12.5 to 100μg/ml inhibited the proliferative response of lymphocytes to ConA.Additionally,SE(12.5-100μg/ml)inhibited secretion of NO by peritoneal macrophages stimu- lated by LPS or LCS.This study reveals that SE inhibits the activation and proliferation of murine lym- phocytes and NO secretion by peritoneal macrophages.
The aim of this study is to elucidate the molecular and cellular mechanisms underlying the immunosuppressive effect of Sanchi extract(SE)via investigating the effects of SE on the activation and proliferation of murine lymphocytes and NO secretion by peritoneal macrophages in vitro.ConA was used to Activated lymphocytes, and expression of CD69 on T cells and CFSE labeled cell division were detected by flow cytometry.Murine peritoneal macrophages were stimulated with LPS or lymphocytes culture culture (LCS) and the concentration of NO was determined by Griess reagent assay.After 6 h Of culture,SE ranging from 50 to 100μg/ml downregulated CD69 expression on ConA-activated T cells,while SE ranging from 12.5 to 100μg/ml inhibited the proliferative response of lymphocytes to ConA.Additionally,SE(12.5-100μg/ml)inhibited The secretion of NO by peritoneal macrophages stimu-lated by LPS or LCS.This study reveals that SE inhibits the activation and proliferation of murine lym- phocytes and NO secre Tion by peritoneal macrophages.