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目的:研究分析丹酚酸B在大鼠体内的代谢产物及途径。方法:取SD大鼠12只,分为两组。用于收集空白血浆、胆汁、尿液样品的大鼠各2只;尾静脉注射丹酚酸B(100 mg·kg-1)后,用于收集血浆、胆汁、尿液中丹酚酸B代谢产物的大鼠各2只;采用超高效液相色谱串联四极杆飞行时间质谱(UPLC-Q-TOF)联用技术,对处理后的各样品进行检测,并对代谢产物进行定性与结构鉴定分析。结果:根据质谱信息,共检测到大鼠体内丹酚酸B相关代谢产物11个,其中血浆样品中检测到丹酚酸B酯键水解等2个代谢产物;胆汁样品中检测到丹参素、紫草酸和紫草酸的甲基化物等5个代谢产物;尿液样品中检测到丹酚酸B甲基化和硫酸化等4个代谢产物。结论:丹酚酸B可在大鼠体内代谢为丹参素等代谢产物;依据检测的代谢产物,推测丹酚酸B代谢反应位置主要在酯键和五元环上,代谢途径主要有酯键水解、分子内脱水、脱羧、羟化、甲基化和硫酸化等。
OBJECTIVE: To study the metabolites and pathways of salvianolic acid B in rats. Methods: Twelve SD rats were divided into two groups. Two rats were used to collect blank plasma, bile and urine samples respectively. After tail vein injection of salvianolic acid B (100 mg · kg-1), the samples were collected for plasma, bile and urine salvianolic acid B metabolism 2 rats in each group. The products were analyzed by UPLC-Q-TOF combined with ultra performance liquid chromatography (HPLC-MS / MS), and the metabolites were identified qualitatively and structurally analysis. Results: According to the mass spectrometry information, 11 metabolites of salvianolic acid B in rats were detected, of which 2 metabolites such as salvianolic acid B ester bond were detected in plasma samples. Danshensu and Violet Oxalic acid and lithospermic acid methylated five metabolites; urine samples detected salvianolic acid B methylation and sulfated four metabolites. Conclusion: Salvianolic acid B can be metabolized into metabolites such as Danshensu in rats. Based on the detected metabolites, it is speculated that the metabolic reaction of salvianolic acid B is mainly on the ester bond and the five-membered ring, and the metabolic pathway is mainly ester bond hydrolysis , Intramolecular dehydration, decarboxylation, hydroxylation, methylation and sulfation.