证明了小麦 (TriticumaestivumL .)线粒体上存在肌球蛋白。通过免疫印迹鉴定发现小麦线粒体蛋白重链与抗体进行交叉反应 ,其分子量略高于动物骨骼肌肌球蛋白的重链 ,经计算 ,该蛋白的分子量为 2 10kD。通过电镜观察到溶液中的F_肌动蛋白可以和NEM (N_ethylmaleimide)处理的线粒体结合 ,并发现F_肌动蛋白可以激活线粒体悬浮液的ATP酶活性 ,证明线粒体外膜的外表面存在肌球蛋白 It is demonstrated that myosin is present on the mitochondria of wheat (Triticum aestivum L.). Western blot analysis showed that the heavy chain of wheat mitochondrial protein cross-reacted with the antibody, and its molecular weight was slightly higher than that of the skeletal myosin heavy chain. The molecular weight of the protein was 210kD. Electron microscopy showed that F_-actin in solution could bind to N_ethylmaleimide-treated mitochondria and found that F_-actin activated the mitochondrial ATPase activity of the mitochondrial suspension, demonstrating the presence of myosin on the outer surface of the mitochondrial outer membrane protein