【摘 要】
：
Primary dormant seeds of Arabidopsis thaliana did not germinate in darkness at temperature higher than 10–15°C. Ethylene improved the germination of dormant wild-type (Col-0) seeds at 25°C in darkness but seeds of the mutant affected in the proteolytic N-
【机 构】
：
UMR7622 Biologie du Développement,Sorbonne Université,IBPS,CNRS,Paris F-75005,France;Department of C
论文部分内容阅读
Primary dormant seeds of Arabidopsis thaliana did not germinate in darkness at temperature higher than 10–15°C. Ethylene improved the germination of dormant wild-type (Col-0) seeds at 25°C in darkness but seeds of the mutant affected in the proteolytic N-degron pathway, proteolysis6 (prt6), were insensitive to ethylene suggesting that PRT6 was involved in dormancy release by ethylene. The substrates of the N-degron pathway, the Ethylene Response Factors from group VⅡ(HRE1, HRE2, RAP2.2, RAP2.3, and RAP2.12), were identified to be involved in this insensitivity with an increased ger-mination in prt6 rap2.2 rap2.3 rap2.12 rather than in prt6 hre1 hre2, which also indicated that the three RAPs acted downstream of PRT6, while the two HREs acted upstream of PRT6. Ethylene reduced the expression of the three RAPs in Col-0 seeds but they were maintained or induced by ethylene in prt6 seeds. The promoting effect of ethylene was asso-ciated with a down-regulation of dormancy-related genes in gibberellins (GAs) and abscisic acid (ABA) signaling, such as RGA, RGL2, and ABI5, and with a strong decrease in ABA/GA4 ratio in the presence of ethylene. In contrast, we show that the insensitivity of prt6 seeds to ethylene was mainly related to GA signaling disturbance.
其他文献
A cellular automaton-finite element (CA-FE) model was used to predict the solidification grain structure of per-manent mold cast A7086 alloy. In this model, a Gaussian distribution of nucleation sites was adopted, and the Kurz-Giovanola-Trivedi model was
A high-throughput method was applied to study oxidation behavior of Nb–Si based alloy using composition spread alloy film as combinatorial libraries. An extended range of composition gradients of Nb–Si based alloy film was deposited by (multi)magnetron co
Variant selection during phase transformation creates a specific transformation texture and finally affects the mechanical properties of the alloys. The transformation lattice deformation associated selection mechanism has not been well addressed. In the
Although the genetic basis for endosperm devel-opment in maize (Zea mays) has been well studied, the mechanism for coordinating grain filling with increasing kernel size remains elusive. Here, we report that increased kernel size was selected during moder
Efficient genetic transformation has the potential to advance research and breeding in watermelon (Cit-rullus lanatus), but regeneration from tissue culture remains challenging. Previous work showed that expressing a fusion of two interacting transcriptio
Single crystal Ni-based superalloys are the typical structural materials for high-pressure turbine blades, and their microstructure is critical in determining their mechanical properties. The withdrawal rate is a key parameter affecting the microstructure
This research provides a new insight into designing next-generation filtration materials for Nano-pollutants removal from wastewater, and potentially for gas/solid or liquid/solid separation in corrosive environment. Considering the high corrosion and oxi
Grain size and leaf angle are key agronomic traits that determine final yields in rice. However, the underlying molecular mechanisms are not well un-derstood. Here we demonstrate that the Oryza sativa Mitogen Activated Protein Kinase Kinase Kinase OsMKKK7
目的 对牛痘疫苗接种家兔炎症皮肤提取物(神经妥乐平)治疗2型糖尿病(type 2 diabetes)同时合并周围神经病变(DPN)病人的作用效果和药物使用的安全性进行系统评价,为神经妥乐平在2型糖尿病病人同时合并DPN治疗中的合理使用提供循证证据.方法 检索循证图书馆、PubMed文献数据库、EMBASE、万方电子数据库、中国生物医学文献数据库(CBM)、中国期刊全文数据库(CNKI)等,按照筛选标准对神经妥乐平治疗DPN相关的临床随机对照研究(RCT)相关文章进行收集整理,检索时限从各数据库建库起至20
Traditional Chinese medicine (TCM) belongs to the most elaborate and extensive systems of plant-based healing. The herb Northern Ban Lan (Isatis tinctoria) is famous for its antiviral and anti-inflammatory activity. Although nu-merous components isolated