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采用灌胃的方法,对雄性小鼠进行三丁基锡(TBT)染毒,剂量为1mg/(kg.d),然后分别用不同剂量的大蒜素油进行保护。保护组高剂量为200mg/(kg.d),低剂量为100mg/(kg.d),试验7d。试验结果表明:大蒜素油保护组小鼠肝组织活性氧(ROS)活性和丙二醛(MDA)含量均明显低于TBT对照组;电镜观察发现TBT染毒后小鼠胸腺细胞核损伤较为明显,主要是染色质高度凝聚、边缘化,甚至崩解消失,而大蒜素油保护组细胞核损伤明显减轻;彗星试验发现TBT染毒组淋巴细胞尾长和尾相都显著升高,而大蒜素油保护组尾长和尾相与TBT对照组差别明显,并与剂量相关。因此小鼠TBT染毒后给予一定剂量的干预剂——大蒜素油,对TBT引起的细胞核损伤有明显的保护作用。
Male mice were exposed to tributyltin (TBT) at a dose of 1 mg/(kg.d) by gavage and then protected with different doses of allicin oil. The protective group had a high dose of 200 mg/(kg.d), a low dose of 100 mg/(kg.d), and a test of 7d. The results showed that the activities of reactive oxygen species (ROS) and malondialdehyde (MDA) in the liver tissue of allicin oil-protected mice were significantly lower than those in the TBT control group. Electron microscopy revealed that the thymocyte nuclear damage was more pronounced in mice after TBT exposure. The chromatin was highly aggregated, marginalized, and even disintegrated and disappeared, whereas the allicin oil protected group significantly reduced the nuclear damage; the comet assay found that the TBT-exposed group had a significantly increased lymphocyte tail length and tail phase, while the allicin oil protection group tail length The tail phase was significantly different from the TBT control group and related to the dose. Therefore, a certain dose of an intervention agent, allicin oil, was given to the mice after TBT exposure, which has a significant protective effect on the nuclear damage caused by TBT.