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肝脏是人体和动物的重要解毒器官,可作为环境污染物在体内负荷的指示器,肝脏中的元素含量及其赋存状态与人体健康和疾病密切相关.采用差速离心分离技术将正常人肝组织分离为细胞核、线粒体、溶酶体、微粒体和胞液等5个亚细胞组分,结合原子吸收和原子荧光光谱分析研究了As,Cd,Hg,Pb等重金属在正常人肝组织及其分离细胞器组分中的相对分布.结果表明所测重金属总量与已报道的值基本一致.分离亚细胞组分分布表明,Hg在线粒体、微粒体和胞液等组分中浓度较高;Cd在胞液中浓度最高,其次为线粒体;As在细胞核中浓度较高;而Pb在微粒体中浓度很高,且与Fe的分布模式相类似.人肝中汞主要以无机汞形态存在,甲基汞约占总汞的9%~50%,平均为20.9%13.3%.
The liver is an important detoxification organ for human and animals, which can be used as an indicator of the environmental load of the pollutants in the body. The content of the elements in the liver and its state of occurrence are closely related to human health and disease.Using differential centrifugation technology, Tissues were separated into 5 subcellular fractions including nucleus, mitochondria, lysosomes, microsomes and cytosol. The effects of As, Cd, Hg, Pb and other heavy metals on normal human liver tissues and their The results showed that the total amount of heavy metals tested was basically the same as the reported value.The separation of subcellular fractions showed that the concentration of Hg was higher in mitochondria, The highest concentration in the cytosol, followed by the mitochondria; As in the nucleus of the higher concentration; and Pb in the microsomes in a high concentration, and similar to the distribution pattern of Fe Mercury in human liver exists mainly in the form of inorganic mercury, A Basal mercury accounts for about 9% to 50% of total mercury, with an average of 20.9% and 13.3%.