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目的 研究抗氧化剂L 乙酰半胱氨酸是否能够保护脉冲噪声引起的耳聋。方法 第 1组动物 :8只大鼠暴露在16 0dBSPL 5 0次脉冲噪声。第 2组动物 :10只大鼠同样暴露 16 0dBSPL 5 0次脉冲噪声 ,但在噪声暴露前 1h ,噪声暴露后立刻 ,噪声暴露后 3h ,给予L 乙酰半胱氨酸腹腔内注射 (35 0mg·kg-1,IP)。第 3组动物 :5只给予腹腔注射L 乙酰半胱氨酸 3次 ,注射方法与第 2组相同 ,不暴露脉冲噪声。全部动物通过脑干诱发电位检测听力阈值在噪声前 ,噪声后立刻 ,噪声后 1d ,1,2 ,4wk。结果 第 1组 :比较噪声前和噪声后 ,全部频率有明显听力损失。第 2组 :大鼠暴露同样剂量噪声 ,立刻和 1天后检测ABR阈值与暴露前相比听力损失明显 ,1wk后在 4到 2 0kHz都有听力损失 ,但在 31 5和 4 0kHz已不明显。在 2和 4wk之后 ,各频率听力损失不明显。第 3组 :立刻 ,1d后 ,4wk后测量脑干诱发电位各频率无听力损失。结论 抗氧化剂L 乙酰半胱氨酸能够保护脉冲噪声引起的听力损害。
Objective To investigate whether antioxidants L-acetylcysteine can protect against deafness caused by impulse noise. Method Group 1 Animals: Eight rats were exposed to 60 pulses of 60 dB SPL for 50 pulses. Group 2 animals: 10 rats were equally exposed to 60 dB SPL 50 impulse noise, but given 1 h before noise exposure, immediately after noise exposure, and 3 h after noise exposure, L-acetylcysteine was injected intraperitoneally (35 0 mg · kg-1, IP). Animals in Group 3: Five animals were given intraperitoneal injections of L-acetylcysteine 3 times in the same manner as in Group 2 without impulsive noise exposure. All animals through the brain stem evoked potentials to detect hearing threshold in the noise before the noise immediately after noise 1d, 1, 2, 4wk. Results Group 1: After comparing noise before and after noise, all frequencies had significant hearing loss. Group 2: rats exposed to the same dose of noise, immediately and 1 day after the test ABR threshold hearing loss compared with pre-exposure significantly, after 1wk have hearing loss at 4 to 20kHz, but at 31 5 and 4 0kHz is not obvious. After 2 and 4wk, hearing loss at each frequency was not significant. Group 3: immediately, 1d, 4wk after the measurement of brainstem response potential frequency of hearing loss. Conclusion Antioxidant L-acetylcysteine can protect hearing loss caused by impulse noise.