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目的研究镍化合物导致大鼠肺泡巨噬细胞凋亡机制。方法用Ni3S2和Ni2O3对大鼠进行染毒,16个月后收集各组动物的肺泡巨噬细胞,运用流式细胞术(FCM)测定细胞的周期分布、胞内钙离子浓度和线粒体膜电位。结果Ni3S2组大鼠肺泡巨噬细胞较对照组的凋亡率(%)明显增加〔(8.6±5.5)和(2.2±0.7),P<0.05〕,胞内游离钙浓度增加〔(26.6±8.7)和(19.5±0.5),P<0.05〕,线粒体膜电位变小〔(7.7±3.4)和(10.9±0.84),P<0.05〕。Ni2O3组较对照组的凋亡率和胞内游离钙浓度均增加,线粒体膜电位下降。结论线粒体膜电位下降,胞内外Ca2+浓度的改变可能是造成染镍大鼠肺泡巨噬细胞凋亡的原因。
Objective To study the mechanism of nickel compound inducing apoptosis of alveolar macrophages in rats. Methods The rats were exposed to Ni3S2 and Ni2O3. After 16 months, the alveolar macrophages of each group were collected. The cell cycle distribution, intracellular Ca2 + concentration and mitochondrial membrane potential were measured by flow cytometry (FCM). Results The apoptotic rate (%) of alveolar macrophages in the Ni3S2 group was significantly higher than that in the control group [(8.6 ± 5.5) vs (2.2 ± 0.7), P <0.05], and the intracellular free calcium concentration increased [(26.6 ± 8.7 ) And (19.5 ± 0.5), P <0.05〕. The mitochondrial membrane potential decreased (7.7 ± 3.4) and (10.9 ± 0.84), P <0.05. The apoptosis rate and intracellular free calcium concentration of Ni2O3 group increased compared with control group, and the mitochondrial membrane potential decreased. Conclusion The decrease of mitochondrial membrane potential and the change of intracellular Ca2 + concentrations may be responsible for the apoptosis of alveolar macrophages in nickel-exposed rats.