论文部分内容阅读
目的研究茶多酚(TP)对体外培养的人脐静脉内皮细胞(HUVEC)纤溶功能的保护作用。方法甲基噻唑基四唑(MTT)法筛选不影响细胞增殖的TP和Hcy浓度。实验分为同型半胱氨酸(Hcy)0.5mmol/L、TP10μg/ml、Hcy0.5mmol/L+TP10μg/ml与对照共4组,用酶结合免疫吸附测定(ELISA)法检测培养细胞上清液中纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制剂-1(PAI-1)含量,反转录聚合酶链反应(RT-PCR)观察t-PA和PAI-1 mRNA水平。结果与对照组相比,Hcy0.5mmol/L可使PAI-1含量增高6.57%,mRNA水平增高45.7%,差异均有统计学意义(P<0.01);TP与Hcy共同作用后则可使PAI-1含量及mRNA水平较Hcy组分别降低8.8%和25.9%,均有统计学差异(P<0.01)。TP可逆转Hcy引起的t-PA mRNA水平下降,使其增高38.1%(P<0.01),但各组t-PA含量均未见改变;TP可保持PAI-1/t-PA比值接近正常水平。结论TP可通过调节mRNA水平来降低PAI-1含量,使PAI-1/t-PA接近正常水平,从而维持内皮细胞的正常纤溶功能。
Objective To study the protective effect of tea polyphenols (TP) on the fibrinolytic function of cultured human umbilical vein endothelial cells (HUVECs). Methods Methyl thiazolyl tetrazolium (MTT) method was used to screen the concentrations of TP and Hcy which did not affect cell proliferation. The experiment was divided into 4 groups: Hcy 0.5 mmol / L, TP 10 μg / ml, Hcy 0.5 mmol / L + TP 10 μg / ml and control group. The supernatant of cultured cells was detected by enzyme-linked immunosorbent assay (ELISA) (T-PA) and plasminogen activator inhibitor-1 (PAI-1) were detected by RT-PCR and t-PA and PAI- 1 mRNA level. Results Compared with the control group, Hcy0.5mmol / L increased the content of PAI-1 by 6.57% and the mRNA level by 45.7% (P <0.01), while the combination of TP and Hcy increased PAI-1 -1 levels and mRNA levels were reduced by 8.8% and 25.9% respectively in Hcy group (P <0.01). TP could reverse the level of t-PA mRNA induced by Hcy and increase the level of t-PA mRNA by 38.1% (P <0.01), but there was no change in the content of t-PA in all groups. TP maintained the level of PAI-1 / t-PA close to normal . Conclusion TP can reduce the content of PAI-1 by regulating the mRNA level and make PAI-1 / t-PA close to the normal level so as to maintain the normal fibrinolytic function of endothelial cells.