论文部分内容阅读
以大白菜不育材料“939A”为母本,携带恢复基因的材料YDQ56A为父本构建隐性雄性不育F1S4分离群体,利用混合分组分析法(BSA)对不育基因初定位;同时以“939A”为母本,携带保持基因的材料yellow sason为父本构建显性雄性不育F2分离群体,对不育基因进行精细定位。结果在F1S4群体中获得与不育基因连锁的标记2个,A08_1900和Br ID111035,与不育基因分别相距8.8c M和2.5c M,物理距离为804.1kb;F2群体中不育单株与可育单株分离比符合3∶1,不育基因表现为显性。通过标记验证,F2和F1S4两个群体定位结果一致,均位于A08染色体末端,通过新标记的筛选获得不育基因两侧紧密连锁的标记Br19470306和Br19675586,与不育基因分别相距1.6c M和2.4c M,物理距离205.28kb,其中包含58个基因。该结果为大白菜雄性不育系的利用以及转育奠定了基础。
The recessive male sterility F1S4 segregation population was constructed with the male sterile line “939A” as the female parent and YDQ56A harboring the restorer gene as the male parent, and then the hybrid microsatellite loci (BSA) Using male sterile line sisason (yellow sason) with “939A” as the female parent, a dominant male sterile F2 segregation population was constructed to finely locate the sterile gene. The results showed that two markers A081900 and Br ID111035 linked to the sterile gene were obtained in the F1S4 population, separated from the sterile gene by 8.8c M and 2.5c M, respectively, and the physical distance was 804.1kb. The single plant isolation ratio was 3:1, and the sterile gene was dominant. F2 and F1S4 were located at the end of chromosome A08. The markers Br19470306 and Br19675586 closely linked on both sides of sterile gene were obtained by screening the marker, 1.6c M and 2.4 c M, physical distance 205.28kb, which contains 58 genes. The results laid the foundation for the utilization and breeding of Chinese cabbage male sterile lines.