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目的建立一种易于观察、取材方便、价格低、实验周期短、经济实用的鸡胚背根神经节神经元(dorsal root gan-glions neuron,DRGn)体内培养的方法。方法摘取孵化13～15d间鸡胚的背根神经节,经胰蛋白酶消化分离等方法获得纯化的神经元,将其按一定浓度移植于载体的绒毛尿囊膜层后,继续培养。结果 DRGn在载体的绒毛尿囊膜层培养3～5d时,可观察到神经细胞仍存活;培养6～9d时,可观察到神经细胞的生长;培养11～13d时,可观察到神经网络形成。结论鸡胚背根神经节神经元能在鸡胚的绒毛尿囊膜层生长、分化及再聚集并发育成正常神经网络组织。
OBJECTIVE: To establish a method for in vivo culture of chick embryo dorsal root ganglion neuron (DRGn) which is easy to observe, easy to draw, low in price, short in experimental period. Methods The dorsal root ganglion (DRG) of chicken embryo hatching for 13 ~ 15 days was harvested and purified by trypsin digestion. The purified neurons were transplanted into the chorioallantoic membrane of the carrier at a certain concentration, and then cultured. Results DRGn could survive in 3-5 days when cultured in the chorioallantoic membrane layer of the vector, and the growth of nerve cells was observed after culture for 6 ~ 9 days. Neural network formation was observed at 11 ~ 13 days . Conclusion The neurons of chick embryo dorsal root ganglion can grow, differentiate and re-aggregate in chick chorioallantoic membrane and develop into normal neural network.