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以龙眼“大乌圆”和荔枝“三月红”两个品种的幼叶和果实组织为试材 ,采用在细胞核被裂解之前 ,去除细胞质中的酚类物质和蛋白质 ,而后用 SDS裂解细胞核 ,异丙醇和乙醇沉淀染色体 DNA的方法 ,成功地从两种不同组织中提取和纯化了两种果树的染色体 DNA,DNA的得率介于 2 86～ 314μg/g FW之间 ,凝胶电泳显示无明显降解现象 ,适宜作为 PCR模板应用 ,并成功地进行特异性基因扩增
The young leaves and fruit tissues of two varieties of longan, “big black circle” and litchi “March Red” were used as materials to remove the phenolics and proteins in the cytoplasm before the nuclei were lysed, and then the nuclei were lysed by SDS, Isopropyl alcohol and ethanol precipitation chromosomal DNA successfully extracted from two different tissues and chromosome DNA of two fruit trees, DNA yield was between 284 ~ 314μg / g FW gel electrophoresis showed no Obvious degradation phenomenon, suitable as a PCR template application, and successfully carried out specific gene amplification