报道一种分离与定量测定生物合成反应液中谷胱甘肽的方法。该反应以半胱氨酸、甘氨酸和谷氨酸为底物,在葡萄糖、氯化镁和磷酸缓冲液条件下进行,反应液用纸层析分离,实验发现胱氨酸替代半胱氨酸参与反应,并报道了五种成分的R_f值:L-半胱氨酸、甘氨酸、L-谷氨酸、谷胱甘肽和L-胱氨酸。结果表明,在混合溶剂系统中,以酚-水(5:1)分离效果最佳。 A method for the isolation and quantitation of glutathione in biosynthetic reaction solution was reported. The reaction of cysteine, glycine and glutamic acid as substrate in glucose, magnesium chloride and phosphate buffer conditions, the reaction solution was separated by paper chromatography, the experiment found that cystine instead of cysteine ​​involved in the reaction, The Rf values ​​of five components were reported: L-cysteine, glycine, L-glutamic acid, glutathione and L-cystine. The results show that in the mixed solvent system, phenol - water (5: 1) separation is the best.