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目的建立生物样品中利多卡因、罗哌卡因、布比卡因的固相萃取提取方法和气质检测方法。方法采用Oasis HLB固相萃取柱,以GC/MS定性定量。结果生物样品中利多卡因、罗哌卡因、布比卡因平均萃取回收率达74%以上,检测限0.01μg/mL,线性关系良好,相关系数R2=0.990 0以上。结论方法操作简便快速,萃取回收率高,重现性好,可用于实际案件。
OBJECTIVE To establish a solid phase extraction (SPE) extraction method for lidocaine, ropivacaine and bupivacaine in biological samples and to detect its temperament. Methods Oasis HLB solid phase extraction column was used for qualitative and quantitative analysis by GC / MS. Results The average recoveries of lidocaine, ropivacaine and bupivacaine in biological samples were over 74% with a detection limit of 0.01 μg / mL. The correlation coefficient was R2 = 0.990 0. Conclusion The method is simple and rapid in operation, high recovery rate of extraction, good reproducibility, and can be used in practical cases.