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目的 :研究口腔鳞状细胞癌与癌旁上皮中的端粒酶活性与增殖细胞核抗原 (PCNA)表达的关系。方法 :应用人端粒酶RNA原位杂交法及免疫组化SP法对 6 4例鳞癌及 30例癌旁上皮进行检测。结果 :显示端粒酶活性阳性的 2 2例鳞癌和 2例癌旁上皮中PCNA阳性细胞密度为 16 5 .37± 2 .98,端粒酶阴性的 40例鳞癌和癌旁上皮中PC NA阳性细胞密度为 97.33± 2 .42。检验证实端粒酶活性阳性组与阴性组之间PCNA阳性细胞密度有显著差异 (P<0 .0 5 )。结论 :端粒酶活性与PCNA阳性表达有关 ,表达端粒酶活性的细胞多处于高度增殖的状态。
Objective: To investigate the relationship between telomerase activity and PCNA expression in oral squamous cell carcinoma and para-cancerous epithelium. Methods: Sixty-four squamous cell carcinomas and 30 paracancerous epithelium were detected by human telomerase RNA in situ hybridization and immunohistochemical SP method. Results: The positive cell number of PCNA in 22 squamous cell carcinoma and 2 paracancer epithelium showed telomerase activity was 16 5. 37 ± 2. 98, telomerase negative 40 squamous cell carcinoma and paracancer epithelial PC The positive cell density of NA was 97.33 ± 2.42. Test confirmed that telomerase activity positive group and negative group PCNA positive cell density was significantly different (P <0. 05). Conclusion: The telomerase activity is related to the positive expression of PCNA, and the cells expressing telomerase activity are highly proliferative.